HI802 iris® visible spectrophotometer quickly identifies Hanna Instruments vial methods by reading the barcode on reagent vials across multiple methods that support the use of a single-zero measurement. The barcode reader for method identification is removable to allow other methods and cuvette adapters (included) to be used for maximum versatility.

HI802 iris® visible spectrophotometer is portable and allows for measurement in the spectrum of all wavelengths of visible light and not just pre–specified wavelengths. Spectrophotometers work by isolating light at specific wavelengths from white light. This compact meter incorporates a number of features that facilitate both fantastic performance and exceptional usability.

• Supplied with 103 factory methods
• Create up to 100 user methods
• Automatic method identification of vial samples
• Vial barcode reader
• Shared single-zero measurement across multiple vial methods
• 5 cuvette types (16 mm round, 22 mm round, 13 mm vial, 10 mm square, 50 mm rectangular) with automatic detection
• Data storage for 9999 measurements with ability to auto log results
• Simplified data transfer to a PC or Mac
• Field upgradeable firmware
• Rechargeable battery

Method identification

Vial barcode reader
Automatic method identification of barcoded sample vials is an exciting feature of HI802. The HI802 visible spectrophotometer scans an inserted barcoded vial and automatically detects both method type and method range, significantly reducing the risk of errors and aiding measurement procedure.

Vial rotation
Vial rotation during measurement allows for method identification and for a number of absorbance readings. The instrument then converts the readings to concentration units and the result is shown on the LCD display. This rotational averaging of signals during measurement (with the lamp on) ensures improved method accuracy.

Shared single-zero measurement
Use of a single-zero measurement across multiple vial methods where blank correction is done with DI water. This ensures that sample features, rather than instrument changes, are reflected in the instrument’s measurements and contributes to ease of use and stable readings.

Features

Advanced split–beam optical system

In a spectrophotometer the optical system is the heart of the instrument. Ensuring that the optical system is built with the best design and highest quality materials will guarantee accurate readings and a long life for the meter. When developing this meter our research and development team payed special attention to details and combined many small improvements to a typical spectrophotometer design to create a portable meter with unprecedented performance.

Spectral range
The meter features a spectral range of 340nm to 900nm allowing for a wide selection of analytical methods. The flexibility of this range permits compliance with many methods from regulatory organizations and associations for a variety of applications.

Replaceable tungsten–halogen lamp
To be able to measure in a wide variety of wavelengths a broadband light source is necessary. In the iris® visible spectrophotometer, this is accomplished by a tungsten–halogen lamp. As these lamps do not last indefinitely, it is necessary to change them throughout the life of the meter. The pre–alignment of the lighting fixture guarantees that the bulb is in the same position every time it is changed. This generates peace of mind as there is no need to worry about realigning the light source.

Concave grating
This element of the optical system is what generates the spectrum of light. When the light from the tungsten lamp hits the grating it is met with interference coatings that turn the polychromatic white light into a rainbow. This rainbow contains dispersed light at all wavelengths in the visible spectrum. The rotation of this grating is what allows for a specific wavelength to be selected. This ability is one of the biggest differences between a spectrophotometer and a photometer. The concave grating which accomplishes this is superior to other types of diffraction, such as prisms, as it minimizes stray light generated and has constant bandwidth. It also combines elements of the optical system that would typically be separate, for example if a flat grating was used a concave mirror would need to be added in order to refocus the light. The combination of these two pieces creates greater efficiency and a smaller optical system to yield a more compact portable meter.

Beam splitter
The beam splitter is added to the optical system for use with a reference detector to ensure that the measurement compensates for any drift in the light source. It works by splitting the light emitted by the tungsten lamp into two beams and sending one beam of light to the reference detector that measures intensity. If there are any fluctuations in the light source the meter detects this and compensates through a mathematical calculation. The reference detector also saves battery life and leads to improved speed of the meter as the lamp doesn’t have to warm up prior to use.

Narrow bandwidth and high resolution
Having a small bandwidth is necessary to accurately measure narrow peaks. The iris® visible spectrophotometer maintains a narrow bandwidth of 5 nm resulting in good spectral resolution. This leads to accurate measurement of sharp, narrow absorbance peaks. Additionally, the high resolution of 1 nm generates greater sensitivity as the wavelength is closer to where the sample absorbs the most light.

System check
Upon turning on the meter a performance check occurs to confirm that the light source is working properly and to calibrate the position of the grating. The grating calibration works by scanning for the “zero order” light reflecting off the grating. If any mechanical problems are present, the meter will display an alert. This feature establishes confidence in measurements knowing that the meter is always working properly without needing to run any additional tests.

Low stray light
A common problem in spectrophotometers is stray light. Stray light can be light which is outside the wavelength the meter is measuring or also light at the proper wavelength but from outside the meter. This leads to inaccurate readings as this light would not be absorbed by the sample but would still be detected by the meter. This is a problem that is typically hard to control. Due to the design of the optical system we are able to keep this potential issue to a minimum to improve the linearity and accuracy of readings.

Universal cuvette holder and auto–recognition
The built-in cuvette holder holds both 22 mm round cuvettes and rectangular cuvettes with a 5 cm path length. Adapters for the cuvette holder are available to hold other 13 and 16 mm round cuvettes, and 10 mm square cuvettes including the 13 mm vial adapter with barcode reader. Rectangular cuvettes have longer path lengths which result in higher sensitivity in readings of low absorbance samples. Additionally, the meter permits the selection of the size of the cuvette used in custom user methods from the available sizes. For all methods, the programmed cuvette size is displayed on the screen to assure that the proper path length is being used by the meter when calculating measurements.

User interface
No one likes to work with difficult equipment, which is why we have worked hard to create an interface that makes the meter’s operation seamless. The intuitive menu design and large LCD screen all make working with the meter a breeze. Get ready for your new favorite piece of lab equipment.

Favorite methods
Always have your most frequently used methods readily available with the favorite methods feature. Directly from the home screen is access to user–programmed favorite methods, saving time.

Large high-contrast custom LCD display
With a 6” display, the screen is large and easy to read. The high contrast makes every character on the display stand out even during outdoor use. The wide viewing angle allows for measurements to be seen from far away, so while working around the lab it is not necessary to hover over the meter to see the measurements.

Capacitive touchpad (for glove use)
Navigating the menus and using the meter is effortless with the capacitive touchpad. Featuring dedicated buttons specifically for setup, logging data, recalling data, and methods allows for quick and easy access to these functions. There is a key beep feature that can be enabled or disabled, for audible feedback that the key was pressed.

Customized methods
The HI802 intuitive user interface guides users step-by-step through the process of creating custom methods i.e.: naming your method, setting the measurement wavelengths, creating reaction timers, and calibrating the method. Up to 10 points can be used to calibrate methods.

• Step–by–step method creation
• Up to 10 calibration points
• Flexible calculations for multi wavelength methods

Pre–programmed methods
Programmed in the meter are more than 100 commonly used methods for chemical analysis. Methods can easily be updated by transferring the file from a computer to the meter or by a flash drive. Up to 150 factory methods can be saved in the meter and some chemical parameters have the option to switch between different chemical forms. Finding the product codes to order additional reagents is easy as the meter provides the appropriate reagent codes for each programmed method.

User methods
The ability to program up to 100 personal methods into the meter creates both versatility and customization. Methods can include up to 10 calibration points, 5 different wavelengths
(which can be used simultaneously), and permits the use of 5 reaction timers. These features allow for many variations to be implemented into methods. Compared to a photometer there is no longer a limitation by factory methods. If a certain parameter is not offered or a modification to a pre–programmed method is required, the meter can be customized to suit your needs.

General Features

When choosing a piece of equipment making sure the product has all required features for the intended purposes is critical. When building the iris® we included as many features as we could to aid in making this meter versatile and convenient. From power features such as long battery life and easy data logging and transfer, we have pushed the limits on seemingly basic features to make laboratory work as easy as possible.

Data logging and transfer
The HI802 can store up to 9999 measurements in memory. Transferring data from a meter should always be simple and straightforward. At any time data can be transferred to a PC or Mac as either a CSV or PDF file. No software is required, simply plug in a flash drive or plug it into a computer and export the data. Additionally, a meter ID and a sample ID can be programmed to be saved along with logged measurements. With technical equipment, wide–spread connection compatibility can often be an issue, which is why the iris® visible spectrophotometer features USB ports for both flash drive and a direct computer connection. Connectivity with a USB–A port to a flash drive can be used to transfer logged measurements from the meter and also to transfer method updates onto the meter. The USB–B port is used for a direct connection to a computer specifically for transferring logged data.

Battery operated
The meter features a rechargeable lithium ion battery that lasts for approximately 3,000 measurements (excl. vial rotation). Lasting well over a day of use in the field there is no need to worry about the battery life while out working without a power supply. The meter can be quickly recharged with a dedicated fast charging adapter.

Primary Applications:
Laboratory, COD